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Office of Neuroscience Research > Neuroscience Calendar > BME Seminar: James Fitzpatrick (WashU Cell Biology & Physiology/Neuroscience)

BME Seminar: James Fitzpatrick (WashU Cell Biology & Physiology/Neuroscience)

“Correlative Optical, X-Ray and Electron Microscopy in study of Disease Pathogenesis”

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When: Thursday, November 02, 2017 at 10:10 AM to 11:00 AM
Where: Whitaker Hall 218 (Danforth Campus)

Abstract: Correlative Microscopy is a recent concept in cellular imaging to extract spatial information across multiple length scales by using different, yet complementary imaging modalities. In its simplest form, Correlated Light and Electron Microscopy (CLEM) couples the multiplexed detection ability of fluorescence microscopy with the nanoscale ultrastructural resolution of electron microscopy. Recent efforts in the group have focused on the development of a targeted CLEM approach to study the pathogenesis of urinary tract infection, in particular focusing on the identification and structural characterization of intracellular bacterial communities (IBC). In contrast, our recent development of Correlated X-Ray and Focused Ion Beam Nanotomgraphy allows the spatial targeting, in three-dimensions, of tissue specific structures in a whole organism for 3D ultrastructural imaging using Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM). We have applied this novel approach to characterize defects in the elastic connective tissue of the heart muscle in a zebrafish model of dilated cardiomyopathy.

Biography: James Fitzpatrick, Ph.D., joined the School of Medicine on June 1st 2015 as the inaugural Scientific Director of the Washington University Center for Cellular Imaging (WUCCI) and as an Associate Professor of Anatomy & Neurobiology and Cell Biology & Physiology. Prior to his appointment, he was Senior Director of Biophotonics and Strategic Technology Initiatives at the Salk Institute for Biological Studies in La Jolla, CA.

Dr. Fitzpatrick completed his undergraduate studies in Chemistry at Kings College, London and undertook graduate training in optical physics at the University of Bristol also in the United Kingdom. During his Ph.D. he designed and developed a novel injection seeded optical parametric oscillator (OPO) laser system for the study of nuclear hyperfine structure in the excited electronic states of gas phase free radical species. After completing his doctorate at the University of Bristol, he moved to the United States as a post-doctoral fellow at the University of Pittsburgh in Pennsylvania. There he shifted his focus to study biological molecules such as peptide mimics and their micro-solvated clusters in the gas-phase using high-resolution fluorescence spectroscopy. In his second post-doc at Carnegie Mellon University, also in Pittsburgh, he spent his time studying protein-protein interactions using tools such as fluorescence microscopy and fluorescence correlation spectroscopy (FCS). After completing his post-doctoral training, he joined the Carnegie Mellon National Technology Center for Networks and Pathways, an NIH funded Roadmap initiative whose mandate was to develop fluorescent probe and imaging informatics technologies to study networks and pathways in living cells. Dr. Fitzpatrick’s primary research interests lie in the integration and application of multi-scale optical and charged particle imaging technologies -- specifically, biological applications of ion microscopy, development of correlative 3D light and electron microscopy approaches, and new computational tools to visualize and manipulate large-scale multi-dimensional datasets.



November 6th at 11:00 am - Elizabeth Wayne, room 100, Whitaker Hall

November 16th at 10:10 am - Princess U II Imoukhuede, room 218, Whitaker Hall

December 4th at 11:00 am - Julie C. Liu, room 206, Crow Hall, co-sponsored with the Institute of Materials Science and Engineering


For the complete list of Biomedical Engineering (BME) seminars, click here.

For inquiries contact Karen Teasdale.



Please know that there have been changes to parking on the Danforth Campus due to the east end construction.

Metrolink or biking to the Danforth campus are the easiest options.  

If you choose to drive, the closest parking is in Millbrook garage off of Forest Park Parkway and Throop Drive.  It will take approx. 15 minutes to walk to our building.  Visitor passes can be purchased in The Knight Center on Throop Drive.

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