“Large-scale, high-speed volumetric microscopy and its ophthalmic applications”
Abstract: Optical microscopy is rapidly developing in recent years, and plays an important role in life science and medicine. Light sheet microscopy is one unique method by providing high speed 3D imaging to capture kinetic processes in action. Subcellular resolutions with several tens of Hertz volume rate has been previously achieved. The challenge is the consistent trade-off between imaging scale and resolution, with consistent amount of data. Typical high-speed imaging volume is limited within 1 mm3, unable to capture dynamics interactions across large length scales. In this talk, we will focus on how to achieve large scale (i.e. several millimeters in a single scan), while still maintaining isotropic 3D cellular resolution. Being able to sample large volume, whole organism 4D imaging on Zebra fish larvae can be achieved, as well as volumetric imaging on large brain slice. We will also introduce a unique application of light sheet microscopy for in vivo retinal imaging, a.k.a, light sheet in the living eye
For inquiries contact Hanru (Haini) Zhang.