Hosted by the WashU Biochemistry & Biophysics Community (WashU BB), a student-led group.

Abstract: Single-molecule orientation-localization microscopy builds upon photoswitching mechanisms used in localization microscopy (SMLM) to measure both the positions and orientations of fluorophores with nanoscale resolution. This 6D information enables biophysicists to visualize both the morphology and organization of biomolecular assemblies simultaneously. We have recently found that it is impossible for polarization-sensitive imaging to distinguish a single rotating (“wobbling”) molecule from a pair of molecules that spatially coincide, but measuring a fluorophore’s absorption and emission dipole moments simultaneously offers a solution to this challenge. I’ll also review our group’s recent use of fluorogenic probes to visualize the dynamic architectures of amyloid aggregates and biomolecular condensates.

Refreshments to follow seminar

For inquiries contact the WashU BB Community.